cfe-lab / cfe-lab/MiCall

Report common T-cell receptor sequences

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#461 2 comments 0 reactions 0 assignees View on GitHub

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enhancement
Dominant language
Python
Stars
21
Forks
11
Avg merge
12h 5m
Merged PRs (30d)
24

Description

After merging the reads and checking for the V3LOOP amplicon, check for T-cell receptor alpha and beta (TCA and TCB) matches. Take all of those matches, and report the most common nucleotide sequences, by exact match. For the first version, report any sequences with prevalence of at least 5% of all T-cell receptor matches.

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First steps

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Research direction

Trace the pipeline from read merging through the V3LOOP amplicon check, then find where TCA and TCB matches are identified and reported. Implement reporting of exact nucleotide-sequence matches whose prevalence is at least 5% of all T-cell receptor matches, and verify the output against representative FASTQ processing results.

Written by the indexing model from the issue text.

Assessment

Tech stack
python
Domain
bioinformatics
Issue type
Feature
Difficulty
4/5
Estimated time
3-5 days
Activity status
Stale
Clarity
Mostly clear
Newbie friendliness
42/100

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