cfe-lab / cfe-lab/MiCall

Investigate using only targeted references

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question
Dominant language
Python
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21
Forks
11
Avg merge
12h 5m
Merged PRs (30d)
24

Description

Since we know which sequences we expect to see in a sample, it might be useful to only use those references for mapping. Then we could use the other references to see what we find in the unmapped sequences.

This task is to take some samples and map them with only the expected references, then compare the results with using all the references. Do we get more noise moving in with the target when we use fewer references, or do we lose more good sequences to the noise when we use all references? Is either approach significantly faster?

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Research direction

Start by identifying the sample set and mapping entry point used by the pipeline. Run mappings with only the expected references and with all references, then compare noise in target and unmapped sequences and measure runtime. Done means the results clearly show whether either approach changes accuracy or speed significantly.

Written by the indexing model from the issue text.

Assessment

Tech stack
python
Domain
bioinformatics
Issue type
Feature
Difficulty
5/5
Estimated time
Over a week
Activity status
Stale
Clarity
Mostly clear
Newbie friendliness
35/100

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