cfe-lab / cfe-lab/MiCall

Map common contaminants

Open
#291 1 comment 0 reactions 0 assignees View on GitHub

Nobody has claimed this yet.

enhancement
Dominant language
Python
Stars
21
Forks
11
Avg merge
12h 5m
Merged PRs (30d)
24

Description

Add a few more references to the preliminary mapping step for things like phiX and E. coli.

Display a graph for each sample showing the relative amounts of target genomes and contaminants. Calculate the portions just based on read counts.

Make the remap step exclude any projects without coordinate references. That way we will calculate the portions of contaminants, but we won't carry them through the remapping and coverage steps.

Vera posted some example code on GitHub.

Contributor guide

No contributing guide indexed for this repository

First steps

  1. Read the whole issue, then the project's contributing guide.
  2. Comment on the issue to say you are picking it up — it saves two people doing the same work.
  3. Fork the repository and make your change on a branch.
  4. Open a pull request that references the issue number.

Research direction

Start by reviewing the preliminary mapping, remap, and coverage stages, then compare them with Vera's example code at github.com/veratai/check_miseq. Done means adding phiX and E. coli references, displaying per-sample read-count proportions, and excluding projects without coordinate references from remapping and coverage.

Written by the indexing model from the issue text.

Assessment

Tech stack
python
Domain
bioinformatics, data
Issue type
Feature
Difficulty
4/5
Estimated time
3-5 days
Activity status
Stale
Clarity
Mostly clear
Newbie friendliness
30/100

Get new issues in your inbox

A short digest of beginner-friendly GitHub issues.