aryarm / aryarm/as_analysis

add support for peak regions for ASOC analysis

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#69 2 comments 0 reactions 0 assignees View on GitHub
Dominant language
R
Stars
10
Forks
9
PR merge metrics
No merged PRs in 30d

Description

- ASOC analysis needs additional BED file for each sample that indicates peak regions found in each sample
- modify `prepare_counts-rna.r` and create a version (`prepare_counts-atac.r`?) that [finds overlaps with peak regions instead of genes ](https://github.com/aryarm/as_analysis/blob/994d3f21201ddea5313c429ea44d46c968f1d905/scripts/prepare_counts-rna.r#L63) and [reads in BED files instead of GTF file ](https://github.com/aryarm/as_analysis/blob/994d3f21201ddea5313c429ea44d46c968f1d905/scripts/prepare_counts-rna.r#L17)

Contributor guide

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Research direction

Start with scripts/prepare_counts-rna.r, especially the input handling and overlap logic linked in the issue. Compare the RNA workflow with the proposed prepare_counts-atac.r design, then verify that each sample's BED file is read and overlaps are found against peak regions rather than genes. Done means the ATAC preparation path produces counts for the requested peak regions.

Written by the indexing model from the issue text.

Assessment

Tech stack
r
Domain
bioinformatics
Issue type
Feature
Difficulty
4/5
Estimated time
3-5 days
Activity status
Stale
Clarity
Mostly clear
Newbie friendliness
38/100

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