ATAC-seq support
- Dominant language
- R
- Stars
- 10
- Forks
- 9
- PR merge metrics
- No merged PRs in 30d
Description
The main challenge with using this pipeline for ATAC-seq data is the use of STAR for mapping within the WASP subworkflow. We could create two new mapping rules within the WASP subworkflow that use `bowtie2` (or `BWA`), instead. And a new config option could be created to switch between the different aligners that we offer.
As for the variant calling pipeline, we could update it to use [VarCA](https://github.com/aryarm/varCA) and have it use different options when executing `BWA`. The only problem with using [VarCA](https://github.com/aryarm/varCA) at the moment is that it doesn't output GQ tags, but issue #60 would resolve that.
Contributor guide
No contributing guide indexed for this repository
Research direction
Start by reviewing the WASP subworkflow's mapping rules and the variant calling pipeline described in the issue. Check the proposed bowtie2/BWA aligner choices, the new configuration option, VarCA's GQ limitation, and issue #60; done means ATAC-seq processing supports the required mapping and variant-calling paths.
Written by the indexing model from the issue text.
Assessment
- Tech stack
- r
- Domain
- bioinformatics
- Issue type
- Feature
- Difficulty
- 5/5
- Estimated time
- Over a week
- Activity status
- Stale
- Clarity
- Needs clarification
- Newbie friendliness
- 25/100