aryarm / aryarm/as_analysis

ATAC-seq support

Open
#61 1 comment 0 reactions 0 assignees View on GitHub
enhancement
Dominant language
R
Stars
10
Forks
9
PR merge metrics
No merged PRs in 30d

Description

The main challenge with using this pipeline for ATAC-seq data is the use of STAR for mapping within the WASP subworkflow. We could create two new mapping rules within the WASP subworkflow that use `bowtie2` (or `BWA`), instead. And a new config option could be created to switch between the different aligners that we offer.

As for the variant calling pipeline, we could update it to use [VarCA](https://github.com/aryarm/varCA) and have it use different options when executing `BWA`. The only problem with using [VarCA](https://github.com/aryarm/varCA) at the moment is that it doesn't output GQ tags, but issue #60 would resolve that.

Contributor guide

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Research direction

Start by reviewing the WASP subworkflow's mapping rules and the variant calling pipeline described in the issue. Check the proposed bowtie2/BWA aligner choices, the new configuration option, VarCA's GQ limitation, and issue #60; done means ATAC-seq processing supports the required mapping and variant-calling paths.

Written by the indexing model from the issue text.

Assessment

Tech stack
r
Domain
bioinformatics
Issue type
Feature
Difficulty
5/5
Estimated time
Over a week
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
25/100

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