DReichLab / DReichLab/EIG

question about smarpca .perl -b parameter

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Description

When I used the smartPca.perl-b parameter, I encountered a question. When the order of the ind file was changed(sort the first or third column, respectively), the PCA results I got were quite different. What caused this? What are the specific requirements for the order of the -b parameter ind files?

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Research direction

Start by reading the smartPca.perl handling of the -b parameter and reproduce the PCA results with the ind file reordered by its first and third columns. Determine the ordering requirements and document the cause of the differing results, including a reproducible example.

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Assessment

Tech stack
perl
Domain
bioinformatics
Issue type
Documentation
Difficulty
4/5
Estimated time
3-5 days
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
25/100

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