AllenInstitute / AllenInstitute/MIES

Ignore symmetric deconvolution timeseries

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Miniature Analysis
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IGOR Pro
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11
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1d 40m
Merged PRs (30d)
8

Description

Analysis of current clamp data with PSX results in deconvolution traces that surpass reasonable detection thresholds but are not associated with actual synaptic events. A feature of these false positives is that the deconvolved traces are symmetric (see image). Would it be possible to filter out these false positives by looking for symmetry in the deconvolved trace? Perhaps a search for an equal opposite sign threshold within a time window would be sufficient.

Open question: should this be an operation where the user can turn it off and adjust other related parameters?

image
blue box: false positive with symmetric deconvolved trace
green box: miniature EPSP (excitatory post-synaptic potential)

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Research direction

Start by examining current-clamp analysis with PSX and the deconvolution traces shown in the issue. Investigate whether symmetric traces can be identified using an opposite-sign threshold within a time window; the operation's configurability and final acceptance criteria remain open questions.

Written by the indexing model from the issue text.

Assessment

Domain
data
Issue type
Feature
Difficulty
5/5
Estimated time
Over a week
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
25/100

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