AlexsLemonade / AlexsLemonade/scRNA-seq_sandbox
Filtering defaults for 10X
- Dominant language
- R
- Stars
- 1
- Forks
- 0
- PR merge metrics
- No merged PRs in 30d
Description
Through working with the Tabula Muris data I found:
1) No filtering of the data: does not work for scran: too many samples need to be dropped because of negative size factors.
2) The default filtering used for smart-seq2 is too stringent. For example: ~17000 samples were filtered down to ~8000, and ~27000 genes were filtered down to ~2000 genes.
### Question to answer:
What's a good starting point for 10X filtering?
Contributor guide
No contributing guide indexed for this repository
Research direction
Start by examining the filtering behavior described for Tabula Muris 10X data and comparing it with the existing smart-seq2 default. Check how the no-filtering case interacts with scran, then document a justified starting point for 10X filtering and the expected effect on samples and genes.
Written by the indexing model from the issue text.
Assessment
- Tech stack
- r
- Domain
- data
- Issue type
- Feature
- Difficulty
- 5/5
- Estimated time
- Over a week
- Activity status
- Stale
- Clarity
- Needs clarification
- Newbie friendliness
- 25/100