AlexsLemonade / AlexsLemonade/OpenScPCA-analysis

Future idea: Use SingleR with SCPCL000822 and SCPCL000824 as references to annotate remaining Ewing samples

Open
#668 0 comments 0 reactions 0 assignees View on GitHub
analysis
Dominant language
HTML
Stars
16
Forks
24
Avg merge
3d 5h
Merged PRs (30d)
4

Description

### If you are filing this issue based on a specific GitHub Discussion, please link to the relevant Discussion.

#292

### Describe the goals of the changes to the analysis module.

I noticed that when using all tumor cells as a reference and celldex references with `SingleR` we get a lot of cells that are tumor cells that have lower expression of marker genes than I would naively expect. I think it's likely that some cells will be more similar to cells from other Ewing samples than to normal cell types in the `celldex` reference, resulting in more cells being called tumor than actually should be.

One thought I had is to create a reference using the two samples that have a good mix of tumor/normal cells in them (SCPCL000822 and SCPCL000824) and use those as a reference with celldex to annotate the rest of the samples in the project.

### What will your pull request contain?

This will probably be at least 2 PRs that will do the following:

- Generate a reference using the annotations from 822 and 824.
- Create a workflow that uses that reference and the `04-run-singler.R` script to run `SingleR` for all other samples in the project. This workflow could probably also use the same template report `02-singler-results.Rmd` to evaluate the results.

### Will you require additional software beyond what is already in the analysis module?

No

### Will you require different computational resources beyond what the analysis module already uses?

No

### If known, when do you expect to file the pull request?

We should do this once we have confidently annotated normal cells in 822 and 824. I also don't know that we need to do this, but I think it could be helpful in refining the annotations that we currently have so I'm filing this as an idea for future analysis.

Contributor guide

Open the contributing guide

Research direction

Review discussion #292 and the existing 04-run-singler.R and 02-singler-results.Rmd files, once annotations for SCPCL000822 and SCPCL000824 are considered reliable. The proposed work is to generate a reference from those samples and create a workflow for the remaining samples. Done means the reference, workflow, and evaluation report are available for assessing the revised annotations.

Written by the indexing model from the issue text.

Assessment

Tech stack
r
Domain
bioinformatics
Issue type
Feature
Difficulty
5/5
Estimated time
Over a week
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
25/100

Get new issues in your inbox

A short digest of beginner-friendly GitHub issues.