AlexsLemonade / AlexsLemonade/OpenScPCA-analysis
Future idea: Use SingleR with SCPCL000822 and SCPCL000824 as references to annotate remaining Ewing samples
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Description
### If you are filing this issue based on a specific GitHub Discussion, please link to the relevant Discussion.
#292
### Describe the goals of the changes to the analysis module.
I noticed that when using all tumor cells as a reference and celldex references with `SingleR` we get a lot of cells that are tumor cells that have lower expression of marker genes than I would naively expect. I think it's likely that some cells will be more similar to cells from other Ewing samples than to normal cell types in the `celldex` reference, resulting in more cells being called tumor than actually should be.
One thought I had is to create a reference using the two samples that have a good mix of tumor/normal cells in them (SCPCL000822 and SCPCL000824) and use those as a reference with celldex to annotate the rest of the samples in the project.
### What will your pull request contain?
This will probably be at least 2 PRs that will do the following:
- Generate a reference using the annotations from 822 and 824.
- Create a workflow that uses that reference and the `04-run-singler.R` script to run `SingleR` for all other samples in the project. This workflow could probably also use the same template report `02-singler-results.Rmd` to evaluate the results.
### Will you require additional software beyond what is already in the analysis module?
No
### Will you require different computational resources beyond what the analysis module already uses?
No
### If known, when do you expect to file the pull request?
We should do this once we have confidently annotated normal cells in 822 and 824. I also don't know that we need to do this, but I think it could be helpful in refining the annotations that we currently have so I'm filing this as an idea for future analysis.
Contributor guide
Research direction
Review discussion #292 and the existing 04-run-singler.R and 02-singler-results.Rmd files, once annotations for SCPCL000822 and SCPCL000824 are considered reliable. The proposed work is to generate a reference from those samples and create a workflow for the remaining samples. Done means the reference, workflow, and evaluation report are available for assessing the revised annotations.
Written by the indexing model from the issue text.
Assessment
- Tech stack
- r
- Domain
- bioinformatics
- Issue type
- Feature
- Difficulty
- 5/5
- Estimated time
- Over a week
- Activity status
- Stale
- Clarity
- Needs clarification
- Newbie friendliness
- 25/100